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Plasminogen Activator Assay Kits

iD Sensolyte® Assay Kits

Urokinase-type plasminogen activator (uPA) is a serine protease that functions in the conversion of the zymogen plasminogen to the active, broad-spectrum serine protease plasmin. Plasmin, in turn, mediates the pericellular proteolysis of extracellular matrix components and activates other proteases such as matrix metalloproteinases and collagenases that lead to the further degradation and remodeling of the extracellular matrix. Besides the proteolytic function, upon binding to uPA receptor (uPAR), uPA is involved in initiating versatile intracellular signal pathways that regulate cell proliferation, adhesion, and migration through its interaction with various integrins and vitronectin. uPA has been implicated in a number of normal and pathological processes, and recognized as a prognostic marker for various cancers as well as a target for drug discovery. 

Tissue-type plasminogen activator (tPA) is a serine protease that cleaves pro-enzyme plasminogen into active plasmin. It has been recognized that tPA play important roles in various physiological and pathological processes. In plasma, it functions as a thrombolytic enzyme and can be used for treatment of ischemic stroke. In the central nervous system, tPA promotes events associated with synaptic plasticity and cell death in a number of settings, such as cerebral ischemia and seizures. 

The SensoLyte® AFC Urokinase (uPA) Assay Kit is optimized for screening of enzyme inhibitors. This kit contains a fluorogenic substrate with a high reactivity and low background. uPA cleaves the substrate resulting in release of AFC (7-amino-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation /emission= 380/500 nm. 

The SensoLyte® AMC tPA Activity Assay Kit is optimized for assaying tPA enzyme activities. This kit contains a fluorogenic substrate with a high reactivity and low background. tPA cleaves the substrate resulting in release of AMC (7-amino-4-methylcoumarin) fluorophore. Fluorescence can be monitored at excitation /emission= 354/442 nm. This kit can be used for high throughput screening of tPA inducers and inhibitors.

DescriptionSize Reference USD Qty  
SensoLyte® AFC Urokinase (uPA) Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72159 456.00Add to cart
SensoLyte® AMC Tissue-type (tPA) Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72160 456.00Add to cart


Description



λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Sensolyte® AFC Urokinase
Fluorimetric
380/500 5-50µl 0,156 µM • uPA substrate
• AFC fluorescence reference standard
• 2X Assay buffer
• Puridied human uPA
• uPA inhibitor
1 hour 100
Sensolyte® Green
Elastase
Fluorimetric
354/442 5-50µl 0,32 µM • tPA substrate
• AMC fluorescence reference standard
• 2X Assay buffer
• Puridied human uPA
• uPA inhibitor
1 hour 500
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