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Sortase Assay Kits

iD Sensolyte® Assay Kits

Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria.(1) Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria.(2) Since sortases are widely distributed among a variety of bacterial pathogens and required for virulence, they represent a promising therapeutic target for the development of novel anti-infective agents.(3) In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation.(4)

DescriptionSize Reference USD Qty  
SensoLyte® 520 Sortase A Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72228 501.00Add to cart


Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria.(1) Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria.(2) Since sortases are widely distributed among a variety of bacterial pathogens and required for virulence, they represent a promising therapeutic target for the development of novel anti-infective agents.(3) In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation.(4)

The SensoLyte® 520 Sortase A Assay Kit employs a novel internally quenched 5-FAM/QXL® FRET substrate for the detection of protease activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.


Benefits



• Optimized Sensitivity: - Highly sensitive fluorimetric assays
• Bulk reagents: - Ample reagents for 100 assays in 96-well plate format
• High Speed: - Process completed in one hour
• Assured Reliability: - Detailed protocol and references


Description


λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Sortase A
SensoLyte® 520 Sortase A Assay Kit Fluorimetric 490/520 50 µl n.d • 5-FAM/QXLTM520 Sortase substrate,
• 5-FAM, fluorescence reference standard,
• Recombinant human Sortase A
• 2X Assay buffer
• Sortase Inhibitor
30-60 min 100
  • 1. Spirig T, et al. Mol Microbiol 82, 1044 (2011).
  • 2. Marraffini LA, et al. Microbiol Mol Biol Rev 70, 192 (2006).
  • 3. Maresso AW, et al. Pharmacol Rev 60, 128 (2008).
  • 4. Proft T. Biotechnol Lett 32, 1 (2010).

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