Select your products and services :
Select your country :
Americas
Europe
Asia and South Pacific
Middle East and Africa

Update your choices | BU : Life Science | Country : USA

Sortase

Custom & Catalog Recombinant Proteins

Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria.1 Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria.2 Since Sortases are widely distributed among a variety of bacterial pathogens and required for virulence, Sortases represent a promising therapeutic target for the development of novel anti-infective agents.3 In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation.4

DescriptionSize Reference USD Qty  
Sortase A protease, recombinant - 10 µg10 µg AS-72229 374.00Add to cart


Size:

  10 µg

Storage:

  Store at -80°C. Avoid multiple freeze/thaw cycles.

Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria.1 Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria.2 Since Sortases are widely distributed among a variety of bacterial pathogens and required for virulence, Sortases represent a promising therapeutic target for the development of novel anti-infective agents.3 In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation.4

Sortase A from Staphylcoccus aureus was expressed in E. coli expression system with a Nterminal His tag. It is comprised of 26-206 amino acids and its MW is 21.7-kDa.

Sortase A is stored in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, and 2.2 mM KCl, 8 mM imidazole, 0.04% Tween 20, and 20 % glycerol.

The purity is >87% as estimated on SDS PAGE.


References:


  1. Spirig T, et al. Mol Microbiol 82, 1044 (2011).
  2. Marraffini LA, et al. Microbiol Mol Biol Rev 70, 192 (2006).
  3. Maresso AW, et al. Pharmacol Rev 60, 128 (2008).
  4. Proft T. Biotechnol Lett 32, 1 (2010).

On our webshop:

Eurogentec webshop is the simplest and easiest way to place your orders including:

All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.


By email:

order@eurogentec.com

  When placing your order, please include the following information:
  client account number telephone number
  contract number / quotation number product description and reference number
  contact person quantity
  delivery address purchase order number
  invoice address    


By contacting your sales representative:

Consult our sales representative list


By contacting our distributors:

Check our complete distributor list

We use cookies to ensure the best experience on our website. By continuing to use this website, you consent to our cookie policy.