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Sample Processing control

Quantitative PCR (qPCR) Reagents

The Sample Processing Control (SPC) is spiked into samples before extraction to monitor poor extraction yield, PCR inhibition, incorrect pipetting or cycling parameters.

SPC are labelled to Yakima Yellow or Cy®5, custom labelling is available on request.

 

DescriptionSize Reference USD Qty  
qPCR Sample Processing Control Cy5-QXL670 - 200 rxn200 rxn RT-SPCC-Q02 288.00Add to cart
qPCR Sample Processing Control (SPC) DNA template - 1000 rxn1000 rxn RT-SPCD-1000 427.00Add to cart
qPCR DNA Extraction and Inhibition Control Yakima Yellow-BHQ1 - 200 rxn200 rxn RT-SPCY-B02 288.00Add to cart
qPCR DNA Extraction and Inhibition Control Yakima Yellow-BHQ1 - 1000 rxn1000 rxn RT-SPCY-B10 1 286.00Add to cart
qPCR DNA Extraction and Inhibition Control Yakima Yellow-TAMRA - 200 rxn200 rxn RT-SPCY-T02 288.00Add to cart
qPCR DNA Extraction and Inhibition Control Yakima Yellow-TAMRA - 1000 rxn1000 rxn RT-SPCY-T10 1 286.00Add to cart

Eurogentec’s Universal Exogenous qPCR Positive Control for TaqMan® Assays is an optimised Taqman control that was designed to distinguish true target negatives from false negatives due to PCR inhibition, incorrect pipetting or cycling parameters.

  • The optimised control can be spiked into samples before extraction (SPC) or before qPCR Assay (IPC) without compromising amplification efficiency of the target sequence.
  • The SPC doesn't match with any sequence routinely found in a lab. 
  • Detected using a Yakima-Yellow® (VIC® equivalent) or a Cy®5-labelled probe and the target template is detected using a FAM-labeled probe.
  • Avoid amplification of endogenous genes.

High quality result achieved in 42' with FAST qPCR MasterMix Low ROX on an ABI Prism® 7500 FAST cycler using a ten times serial dilution of 18S cDNA (blue lines; detected in the FAM channel). (A) Assay performed in multiplex with the internal Positive Control (orange lines;YY-BHQ-1® version, detected in the VIC®/JOE channel). (B) Assay performed in singleplex, without internal Positive Control.

 

Note: Alternatively, the Universal Exogenous qPCR Positive Control may be used in standardised conditions as extraction yield calibrator, template quality sensor or inter-run calibrator.

A given quantity of control can be spiked into samples before extraction. A relative (directly comparing samples) or an absolute (using a dilution curve of the control) quantification is performed after extraction to normalize the extraction yields of the samples.

Quantitative results of the spiked control within the template or within a reference buffer (pure water, reference template…) are compared in order to reject templates where PCR inhibition is high (low quality).

Add a dilution series of the optimised control on each plate and use it to normalise PCR efficiencies between plates (also for cycler to cycler data normalisation).

Literature & Resources

TDS

Download the qPCR DNA Extraction and Inhibition Control Technical Data Sheets now:

MSDS

Download the qPCR Positive Control MSDS:

Certificate of analysis


To obtain the Certificate of Analysis corresponding to your qPCR kit of interest please contact us via email and display the reference code of the qPCR kit and the batch number. Contact us

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