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Thiol Quantitation Assay Kits

iD Sensolyte® Assay Kits

Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators. 

The SensoLyte® Thiol Quantitation Assay Kit utilizes the widely used Elmann’s reagent for colorimetric measurement of thiol concentrations. The sulfhydryl group of thiol reacts with Ellman’s Reagent [5, 5’-dithiobis (2-nitrobenzoic acid)] and generates 2-nitro-5-thiobenzoic acid (TNB) producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to thiol concentration.

 The SensoLyte® ABD-F Thiol Quantitation Assay Kit provides a convenient, sensitive fluorescent assay for measurement of thiol concentrations. 4-fluoro-7-aminosulfonylbenzofurazan (ABD-F), the reagent provided in the kit, generates yellow-green fluorescence upon reaction with thiols. Fluorescence can be monitored at excitation/emission=389 nm/513 nm. 

The SensoLyte® 520 Thiol Quantitation Assay Kit provides a convenient, highly sensitive fluorescent assay for measurement of thiol concentrations. The novel reagent supplied in the kit, upon reaction with thiols, generates fluorescence which can be easily detected at excitation/emission=490/520 nm. The assay has a linear range of 0.04-160 µM thiol.

DescriptionSize Reference USD Qty  
SensoLyte® 520 Thiol Quantitation Assay Kit #Fluorimetric# - 1 kit1 kit AS-72138 413.00Add to cart
SensoLyte® ABD-F Thiol Quantitation Assay Kit #Fluorimetric# - 1 kit1 kit AS-72137 339.00Add to cart
SensoLyte® Thiol Quantitation Assay Kit #Colorimetric# - 1 kit1 kit AS-72136 286.00Add to cart


λ
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Thiol compounds
SensoLyte® 520 Thiol Quantitation Assay Fluorimetric Ex/Em
490/520
1-10 µl 0.04 μM with
a linear range up to 160 μM
• Fluorimetric Thiol Detection Reagent (490 nm/520 nm)
(Patent pending)
• Reduced Glutathione Standard Stock Solution
• Assay Buffer
30 min 500
SensoLyte® ABD-F Thiol Quantitation Assay Fluorimetric Ex/Em
389/513
1-10 µl 0.25 µM

• ABD-F (4-Fluoro-7-aminosulfonylbenzofurazan) Thiol Detection Reagent (389 nm/513 nm)
• Reduced Glutathione Standard Stock Solution
• Assay Buffer

30 min 500
SensoLyte® Thiol Quantitation Assay Colorimetric Abs
405 or 415
1-10 µl 1.5 µM • Colorimetric Thiol Detection Reagent (Ellman’s Reagent
[5, 5’-dithiobis (2-nitrobenzoic acid)])
• Reduced Glutathione Standard Stock Solution
• Assay Buffer
30 min 500

 

The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.



You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
  telephone number  

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