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Thrombin Assay Kits

iD Sensolyte® Assay Kits

Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion. 

The SensoLyte® Thrombin Assay Kits are optimized for detecting thrombin activity and for screening of enzyme inhibitors. The assay can be performed in a convenient 96-well or 384-well microplate format. 

The SensoLyte® 520 Thrombin Assay Kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate for thrombin. Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. 

The SensoLyte® AFC Thrombin Assay Kit contains a fluorogenic substrate which shows high reactivity and low background. Thrombin cleaves the substrate resulting in the release of the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation/emission= 380/500nm.

DescriptionSize Reference USD Qty  
SensoLyte® 520 Thrombin Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72129 501.00Add to cart
SensoLyte® AFC Thrombin Activity Assay Kit *Fluorimetric* - 1 kit1 kit AS-72130 434.00Add to cart

Description

• Optimized Sensitivity: - These kits are optimized to detect thrombin activity.
• Bulk reagents: - Sufficient to perform 100 assays in a 96-well plate format
• High Speed: - The entire assay process can be completed in one hour
• Assured Reliability: - A detailed protocol and references are provided


λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Thrombin Assay Kit
SensoLyte® 520 Thrombin Assay Kit Fluorimetric 490/520 50 µl 0.6 ng/well • Thrombin Substrate: 5-FAM/QXL™ 520 FRET Peptide
• 5-FAM, Fluorescence Reference Standard,
• 2X Assay Buffer
• Purified Thrombin Enzyme
• Thrombin Inhibitor
• Stop Solution
1 hour 100
SensoLyte® AFC Thrombin Assay Kit Fluorimetric 380/500 50 µl 0.2 ng/well

• AFC (7-amido-4-trifluoromethylcoumarin) Thrombin Substrate,
• AFC Fluorescence Reference Standard,
• 2X Assay Buffer
• Purified Thrombin Enzyme
• Thrombin Inhibitor
• Stop Solution

1 hour 100

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