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Transglutaminase Activity Assay Kits

iD Sensolyte® Assay Kits

Transglutaminases (TG) catalyze cross-link between glutamyl and lysyl residues. TG could be responsible of numerous neurodegenerative diseases due to their ability to promote protein aggregates.

Both colorimetric and fluorimetric SensoLyte® Transglutaminase Activity Assay Kits are convenient for the high throughput screening of TG modulators and inhibitors.

• Optimized Performance: Optimized to detect activity of human transglutaminase enzyme.
• Enhanced Value: Ample reagents to perform 100 assays in a 96-well format.
• Assured Reliability: Detailed protocol and references are provided.

DescriptionSize Reference USD Qty  
SensoLyte® Red Transglutaminase Assay Kit *Fluorimetric* - 1 kit 1 kit AS-72245 457.00Add to cart
SensoLyte® Transglutaminase Activity Assay Kit *Colorimetric* - 1 kit 1 kit AS-72244 457.00Add to cart


Storage and Handling

  • Store component D at -80°C
  • Store components C, F, G, and J at -20°C
  • Store components A, B, E, H, and I at 4°C
  • Protect components G, H from light
  • Protect component I from fluorimetric assay from light

Other Materials Required (but not provided)

  • Colorimetric assay
    Colorimetric microplate reader: Capable of detecting absorbance at 450 nm.
  • Fluorimetric assay
    Fluorescence microplate reader: Capable of detecting emission at 590 nm with excitation at 545 nm.

Transglutaminases (TG) catalyzes irreversible post-translational modifications of proteins. This large family of enzymes contains at least 9 members including TG 1 to 7, the coagulation factor XIIIa and keratinocyte membrane bound TG form1,2. Under physiological conditions, TG doesn’t catalyze transamination reactions. After stimulation, by Ca2+ for example, activated TG catalyzes the cross-link of a glutaminyl residue with a lysyl residue. Additionally to this one, TG can use mono- and polyamines, -OH groups or water as co-substrate. Moreover, other reactions, important for cell viability are also catalyzed by TG. Because of their ability to promote protein aggregates, TG are probably involved in neurodegenerative diseases including Alzheimer’s disease, Huntington’s diseases, Parkinson’s disease or supranuclear palsy and are now considered as potential drug targets3-5.

SensoLyte® Transglutaminase Activity Assay Kit provides a convenient assay for the high throughput screening of TG modulators and inhibitors. Poly-D-lysine are coated on the plate surface. Biotin-TVQQEL peptide contained in the substrate will be covalently linked to lysine by TG. In the fluorimetric kit, the amount of immobilized biotin is determined using streptavidin-horseradish peroxidase (HRP), 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP), and hydrogen peroxide (H2O2). HRP, in presence of H2O2, oxidizes non-fluorescent ADPH into the strong fluorescent resorufin. Otherwise, colorimetric Kit allows the quantification of immobilized biotin using HRP and TMB substrate. Resorufin fluorescence intensity and colorimetric signal generated by TMB are proportional to TG activity in the sample. Assays are performed in a 96-well microplate format.


References

  • 1. Ricotta M. et.al., World J Biol Chem 1 (5), (2010): 181-187
  • 2. Lesort M. et al., Progr Neurobiol 61, (2010): 439-463
  • 3. Pietsch M. et al., Bioorg Med Chem Let 23, (2013): 6528-6543
  • 4. Verhaar R. et al., Neurochem Intl, 58 (7) (2011): 785-93
  • 5. Hartley D.M. et al, J of Biol Chem 283 (24), (2008): 16790-16800
The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

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orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
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