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UNG-dUTP Mix

qPCR Consumables

E. coli Uracil-N-glycosylase (UNG) hydrolyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases).


Using the dUTP/UNG blend easily convert in a single pipetting any dTTP MasterMix into a carryover prevention compatible kit.

Core kits always contain dUTP/UNG blend. No need to buy extra kit converter.

DescriptionSize Reference USD Qty  
5X dUTP/UNG Additive for dTTP kit (7.5 ml) conversion - 5 x 200 µl5 x 200 µl 05-UTPUNG-020 445.00Add to cart
10X dUTP/UNG Additive for dTTP kit (7.5 ml) conversion - 10 x 200 µl10 x 200 µl 10-UTPUNG-020 866.00Add to cart
dUTP/UNG Additive for dttp Kit Conversion - 150 rxn (40 µl)150 rxn (40 µl) RT-UTPUNG-005 32.40Add to cart
dUTP/UNG Additive for dttp Kit Conversion - 750 rxn (200 µl)750 rxn (200 µl) RT-UTPUNG-020 97.20Add to cart
dUTP/UNG Additive for 5 x MMx Kit Conversion - 1250 rxn (335 µl)1250 rxn (335 µl) RT-UTPUNG-033 155.00Add to cart


Source

Recombinant E. coli UNG purified from an E. coli expressing clone.

Storage

For long term storage UNG/dUTP blend should be stored at -20°C in a constant temperature freezer. When stored under these conditions the reagent is stable for 2 years. Avoid repetitive freeze-thaw cycles.

E. coli Uracil-N-glycosylase (UNG) hydrolyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases).

Procedure

Convert a dTTP MasterMix kit before first use:

    - Thaw the MasterMix and the vial containing dUTP/UNG at RT
    -Add the dUTP/UNG blend to the MasterMix vial as follow:

    • 40 μL into 1.5 mL of 2X MasterMix
    • 40 μL into 0.6 mL of 5X MasterMix
    • 67 μL per 1 mL tube of 5X MasterMix
    • 200 μL per 7.5 mL bottle of 2X MasterMix

    - Gently homogenize the MasterMix by swirling the vial
    - Follow MasterMix storage recommendation for the converted kit


Convert a qPCR reaction into a carryover ready assay (for Core kit):
    - Thaw all required reagents
    - Add 0.25 μL of dUTP/UNG per 20 μL reaction (final volume - refer to the TDS of the corresponding Core kit)
    - Gently homogenize the Mix by pipetting or inverting the tube a few times. Spin down.
    - Start the reaction

 

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