Select your products and services :
Select your country :
Americas
Europe
Asia and South Pacific
Middle East and Africa

Update your choices | BU : Life Science | Country : USA

UNG-dUTP Mix

qPCR Consumables

E. coli Uracil-N-glycosylase (UNG) hydrolyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases).


Using the dUTP/UNG blend easily convert in a single pipetting any dTTP MasterMix into a carryover prevention compatible kit.

Core kits always contain dUTP/UNG blend. No need to buy extra kit converter.

DescriptionSize Reference USD Qty  
5X dUTP/UNG Additive for dTTP kit (7,5ml) conversion5 x 200 µL 05-UTPUNG-020 399.00Add to cart
10X dUTP/UNG Additive for dTTP kit (7,5ml) conversion10 x 200 µL 10-UTPUNG-020 775.00Add to cart
dUTP/UNG Additive for dttp kit conv.40 µl - 150 rxns RT-UTPUNG-005 28.10Add to cart
dUTP/UNG Additive for dttp kit conv.200 µl - 750 rxns RT-UTPUNG-020 84.30Add to cart
dUTP/UNG Additive for 5X MMx kit conversion335 µL - 1250 rxns RT-UTPUNG-033 135.00Add to cart


Source

Recombinant E. coli UNG purified from an E. coli expressing clone.

Storage

For long term storage UNG/dUTP blend should be stored at -20°C in a constant temperature freezer. When stored under these conditions the reagent is stable for 2 years. Avoid repetitive freeze-thaw cycles.

E. coli Uracil-N-glycosylase (UNG) hydrolyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases).

Procedure

Convert a dTTP MasterMix kit before first use:

    - Thaw the MasterMix and the vial containing dUTP/UNG at RT
    -Add the dUTP/UNG blend to the MasterMix vial as follow:

    • 40 μL into 1.5 mL of 2X MasterMix
    • 40 μL into 0.6 mL of 5X MasterMix
    • 67 μL per 1 mL tube of 5X MasterMix
    • 200 μL per 7.5 mL bottle of 2X MasterMix

    - Gently homogenize the MasterMix by swirling the vial
    - Follow MasterMix storage recommendation for the converted kit


Convert a qPCR reaction into a carryover ready assay (for Core kit):
    - Thaw all required reagents
    - Add 0.25 μL of dUTP/UNG per 20 μL reaction (final volume - refer to the TDS of the corresponding Core kit)
    - Gently homogenize the Mix by pipetting or inverting the tube a few times. Spin down.
    - Start the reaction

 

The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.



You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
  telephone number  

We use cookies to ensure the best experience on our website. By continuing to use this website, you consent to our cookie policy.