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Universal primers

Oligonucleotides

Nucleic acid sequence analysis is an extensively applied method in Genomics studies. Universal primers are complementary to nucleotide sequences that are very common in a particular set of DNA molecules and cloning vectors. Thus, they are able to bind to a wide variety of DNA templates.

During sequencing, primers are annealed to the denatured DNA template to provide an initiation site for the elongation of the new DNA molecule. Primers can either be specific to a particular DNA nucleotide sequence or they can be “Universal.”

All Universal Oligonucleotitdes (see table just below) are provided RP-HPLC purified and MALDI -TOF MS controlled. They are sent dried in 2 ml tube with a typical final yield of 1 OD / 5 nmol next day after order receipt (subject to instock availability).

DescriptionSize Reference USD Qty  
16S rRNA For (AGA GTT TGA TCC TGG CTC AG)5 nmol UN-PR001-005 9.55Add to cart
16S rRNA Rev (ACG GCT ACC TTG TTA CGA CTT)5 nmol UN-PR005-005 9.55Add to cart
3' RACE PCR (GGC CAC GCG TCG ACT AGT AC)5 nmol UN-PR010-005 9.55Add to cart
Anchored Oligo dT (20) (TTT TTT TTT TTT TTT TTT TV)5 nmol UN-PR015-005 9.55Add to cart
Anchored Oligo dT (22) (TTT TTT TTT TTT TTT TTT TTV N)5 nmol UN-PR020-005 9.55Add to cart
Bluescript KS (TCG AGG TCG ACG GTA TC)5 nmol UN-PR025-005 9.55Add to cart
Bluescript SK (CGC TCT AGA ACT AGT GGA TC)5 nmol UN-PR030-005 9.55Add to cart
cDNA Cloning Primer (GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TV)5 nmol UN-PR035-005 9.55Add to cart
EGFP-C (CAT GGT CCT GCT GGA GTT CGT G)5 nmol UN-PR040-005 9.55Add to cart
EGFP-N (CGT CGC CGT CCA GCT CGA CCA G)5 nmol UN-PR045-005 9.55Add to cart
G3PDH For (ACC ACA GTC CAT GCC ATC AC)5 nmol UN-PR050-005 9.55Add to cart
G3PDH Rev (TCC ACC ACC CTG TTG CTG TA)5 nmol UN-PR055-005 9.55Add to cart
M13 Forward (-20) (GTA AAA CGA CGG CCA GT)5 nmol UN-PR060-005 9.55Add to cart
M13 Forward (-41) (CGC CAG GGT TTT CCC AGT CAC GAC)5 nmol UN-PR065-005 9.55Add to cart
M13 Reverse (-27) (CAG GAA ACA GCT ATG AC)5 nmol UN-PR070-005 9.55Add to cart
M13 Reverse (-48) (AGC GGA TAA CAA TTT CAC ACA GG)5 nmol UN-PR075-005 9.55Add to cart
Neomycin For (CTT GGG TGG AGA GGC TAT TC)5 nmol UN-PR080-005 9.55Add to cart
Neomycin Rev (AGG TGA GAT GAC AGG AGA TC)5 nmol UN-PR085-005 9.55Add to cart
Oligo dT 15 Bases (TTT TTT TTT TTT TTT)5 nmol UN-PR090-005 9.55Add to cart
Oligo dT 16 Bases (TTT TTT TTT TTT TTT T)5 nmol UN-PR095-005 9.55Add to cart
Oligo dT 18 Bases (TTT TTT TTT TTT TTT TTT)5 nmol UN-PR100-005 9.55Add to cart
Oligo dT 20 Bases (TTT TTT TTT TTT TTT TTT TT)5 nmol UN-PR105-005 9.55Add to cart
PCMV Forward (CGC AAA TGG GCG GTA GGC GTG)5 nmol UN-PR110-005 9.55Add to cart
pET 3' (CTA GTT ATT GCT CAG CGG)5 nmol UN-PR115-005 9.55Add to cart
pET 5' (T7) (TAA TAC GAC TCA CTA TAG G)5 nmol UN-PR120-005 9.55Add to cart
pET Upstream (ATG CGT CCG GCG TAG A)5 nmol UN-PR125-005 9.55Add to cart
pGEX 3' (CCG GGA GCT GCA TGT GTC AGA GG)5 nmol UN-PR130-005 9.55Add to cart
pGEX 5' (GGG CTG GCA AGC CAC GTT TGG TG)5 nmol UN-PR135-005 9.55Add to cart
ROSA26 Promoter For (AAA GTC GCT CTG AGT TGT TAT)5 nmol UN-PR140-005 9.55Add to cart
ROSA26 Promoter Rev (GGA GCG GGA GAA ATG GAT ATG)5 nmol UN-PR145-005 9.55Add to cart
SP6 Promoter (TAC GAT TTA GGT GAC ACT ATA G)5 nmol UN-PR150-005 9.55Add to cart
SP6 Upstream (ATT TAG GTG ACA CTA TAG)5 nmol UN-PR155-005 9.55Add to cart
T3 Promoter (AAT TAA CCC TCA CTA AAG GG)5 nmol UN-PR160-005 9.55Add to cart
T7 Promoter (TAA TAC GAC TCA CTA TAG GG)5 nmol UN-PR165-005 9.55Add to cart
T7 Terminator (GCT AGT TAT TGC TCA GCG G)5 nmol UN-PR170-005 9.55Add to cart

Length

  From 15 to 38 bases

Synthesis scale

  40 nmol

Backbone

  DNA

Modification

  None

Purification

  RP-HPLC

Quality Control

  MALDI-TOF MS

Format

  Dried

Packaging

  2 ml tube

Documentation

  Technical Data Sheet

Shipping

  At Room Temperature
The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

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You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
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