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Viral Proteases Assay Kits

iD Sensolyte® Assay Kits

Viral proteases are regarded as important targets for drug discovery against viral diseases. Effectively, they are important in the process of viral particle maturation by allowing cleavage of viral polyproteins.

In Human immunodeficiency virus-1 (HIV-1), the cleavage of the precursor polyproteins, Prgag and Prgag-pol1 by aspartic protease is essential to give mature HIV infectious particles1.

In hepatitis C virus (HCV), NS3/4A is required for the cleavage of viral nonstructural polyprotein at the NS3-NS4A, NS4A-NS4B, NS4B-NS5A, and NS5A-NS5B sites. For West Nile virus (WNV), the NS3 protease activity is absolutely essential to provide structural and functional viral proteins2-8.

The Tobacco Etch Virus (TEV) proteinase recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. This proteinase is widely used to remove fusion tags from recombinant proteins.

DescriptionSize Reference USD Qty  
SensoLyte® 440 West Nile Virus Protease Assay Kit *Fluorimetric* - 1 kit1 kit AS-72079 501.00Add to cart
SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric* 200 assays - 1 kit1 kit AS-72087 457.00Add to cart
SensoLyte® 490 HCV Protease Assay Kit *Fluorimetric* 500 assays - 1 kit1 kit AS-71126 631.00Add to cart
SensoLyte® 490 HIV Protease Assay Kit *Fluorimetric* - 1 kit1 kit AS-71127 631.00Add to cart
SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric* - 1 kit1 kit AS-71145 457.00Add to cart
SensoLyte® 520 HIV Protease Assay Kit *Fluorimetric* - 1 kit1 kit AS-71147 457.00Add to cart
SensoLyte® 520 TEV Protease Assay Kit *Fluorimetric* - 1 kit 1 kit AS-72227 394.00Add to cart
SensoLyte® 570 West Nile Virus Protease Assay Kit *Fluorimetric* - 1 kit1 kit AS-72080 501.00Add to cart


Viral proteases are regarded as important targets for drug discovery against viral diseases. Effectively, they are important in the process of viral particle maturation by allowing cleavage of viral polyproteins.

In Human immunodeficiency virus-1 (HIV-1), the cleavage of the precursor polyproteins, Prgag and Prgag-pol1 by aspartic protease is essential to give mature HIV infectious particles1.

In hepatitis C virus (HCV), NS3/4A is required for the cleavage of viral nonstructural polyprotein at the NS3-NS4A, NS4A-NS4B, NS4B-NS5A, and NS5A-NS5B sites. For West Nile virus (WNV), the NS3 protease activity is absolutely essential to provide structural and functional viral proteins2-8.

The Tobacco Etch Virus (TEV) proteinase recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. This proteinase is widely used to remove fusion tags from recombinant proteins.


Benefits



• Optimized Sensitivity: - Highly sensitive fluorimetric assays
• Bulk reagents: - Convenient mix-and-read format
• High Speed: - One-step homogeneous assays
• Assured Reliability: - Optimized for the most popular fluorescence detection filter channels


Description


λ
Ex/Em
(nm)
Biological sample volume Lowest Detection Limit  Kit contains Assay time # of
assays in
96-well plate
Human immunodeficiency virus (HIV)
SensoLyte® 620 HIV Assay Kit Fluorimetric 590/620 50 µl n.d • HIV-1 Prgag and Prgag-pol proteases substrate: HiLyte™ FluorTR/QXL™610 FRET peptide
• HiLyte™ FluorTR, fluorescence reference standard
• Pepstatin A, a characterized HIV-1 protease inhibitor
• 2X Assay buffer
• Stop solution
• DMSO
• DTT
30-60 min 100
SensoLyte® 520 HIV Assay Kit Fluorimetric 490/520 50 µl n.d

• HIV-1 Prgag and Prgag-pol proteases substrate: HiLyte™ Fluor 488/QXL™520 FRET peptide
• HiLyte™ Fluor488, fluorescence reference standard
• Pepstatin A, a characterized HIV-1 protease inhibitor
• 2X Assay buffer
• Stop solution
• DMSO
• DTT

30-60 min 100
SensoLyte® 490 HIV Assay Kit
Fluorimetric
340/490 50 µl n.d • HIV-1 Prgag and Prgag-pol proteases substrate: EDANS/DABSYL FRET peptide
• EDANS, fluorescence reference standard
• Pepstatin A, a characterized HIV-1 protease inhibitor
• 2X Assay buffer
• Stop solution
• DMSO
• DTT
30-60 min 500
Hepatitis C virus (HCV)
SensoLyte® 620 HCV Assay Kit
Fluorimetric
590/620 50 µl n.d. • HCV NS3/4A protease substrate: HiLyte™ FluorTR/QXL™610 FRET peptide
• HiLyte™ FluorTR, fluorescence reference standard
• Pep4AK HCV NS3 protease cofactor
• 2X Assay buffer
• Stop solution
• DMSO
• DTT
30-60 min 100
SensoLyte® 520 HCV Assay Kit
Fluorimetric
490/520 50 µl 0.1 pmole • HCV NS3/4A protease substrate substrate: 5-FAM/QXL™520 FRET peptide
• 5-FAM, fluorescence reference standard
• Pep4AK HCV NS3 protease cofactor
• 2X Assay buffer
• Stop solution
• DMSO
• DTT
30-60 min 100
SensoLyte® 490 HCV Assay Kit
Fluorimetric
340/490 50 µl 1 pmole • HCV NS3/4A protease substrate substrate: EDANS/DABSYL FRET peptide
• EDANS, fluorescence reference standard
• Pep4AK HCV NS3 protease cofactor
• 2X Assay buffer
• Stop solution
• DMSO
• DTT
30-60 min 500 or 200
West Nile virus (WNV)
SensoLyte® 570 WNV Assay Kit
Fluorimetric
540/575 Up to 85 µl n.d • WNV protease substrate : 5-TAMRA /QXLTM570 FRET peptide
• 5-TAMRA, fluorescence reference standard
• Assay Buffer
• WNV Protease Inhibitor undeca-D-Arg-NH2
30 min 100
SensoLyte® 440 WNV Assay Kit
Fluorimetric
354/442 Up to 85 µl n.d • WNV protease substrate : Pyr-RTKR-AMC
• AMC (7-amino-4-methylcoumarin), fluorescence reference standard
• Assay Buffer
• WNV Protease Inhibitor undeca-D-Arg-NH2
30 min 500
Tobacco Etch Virus (TEV)
SensoLyte® 520 TEV Assay Kit
Fluorimetric
490/520 50 µl n.d • 5-FAM/QXLÔ 520 TEV Substrate,
• 5-FAM, Fluorescence Reference Standard,
• Assay Buffer
• TEV Protease, Recombinant
• DTT
60 min 100
The EGT Ordering System (EOS) is the simplest and easiest way to place your orders including:

 
All oligonucleotides
All Kits & Reagents (including Catalogue Antibodies & Peptides, Real-Time qPCR Mixes, Taq Polymerases, Sensolyte® Assays...)

Please use your Login or Register Now to directly access your customized EOS interface.



You can still place orders by:
orders.anaspec@egt-biotech.com
  When placing your order, please include the following information:
  Contact person purchase order number
  delivery address product description and reference number
  invoice address quotation number
  telephone number  

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