Recombinant protein synthesis is widely used in the life sciences, biotechnology and medicine and can be achieved by cell-based or cell free systems.
Cell-based system is the oldest way to produce large amounts of protein. Host cells are selected depending on the gene involved (post-translational modifications, molecular folding, multi-domain eukaryotic proteins synthesis). There are many different host cells, which may be used for protein production including those derived from bacteria, yeast, baculovirus/insect, and mammalian cells.
In contrast, cell free protein synthesis systems such as PUREfrex™ produce protein in vitro using purified factors necessary for transcription, translation and energy recovery. These systems are suitable to produce in a few hours your protein of interest (e.g. 150 µg DHFR /mL).