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Antibodies against Post-translational modifications

Antibodies against Post-translational modifications

Eurogentec has acquired a strong expertise in producing antibodies against PTMs. We can target classical modifications such as phosphorylation, acetylation, and methylation as well as more complex modifications with high steric hindrance like Ubiquitinylation or SUMOylation.

The Speedy 28-Day programme is particularly adapted for the generation of specific antibodies against PTM due to the high frequency of the injections in this fast programme.

  • Phospho-
  • Acethyl-
  • DiAcetyl-
  • MonoMethyl-
  • DiMethyl-
  • TriMethyl-
  • Oxidised-
  • Citruline-
  • And more

PTM programme step by step

1. Design and synthesis of 2 peptides (1 non-PTM + 1 harbouring the PTM)
2. Coupling of the PTM peptide to a carrier
3. Immunisation of the hosts using the PTM peptide following the Speedy 28-day or a classical 87-day protocol
4. Analysis of the immune response by ELISA and if applicable, selection of the best responding host
5. Double affinity purification of the pAb using the PTM and subsequently the non-PTM peptide

PTM programme purification recognition

After immunisation with the modified peptide, the harvested serum contains a mixture of antibodies recognising the post-translational modification (in blue) and the unmodified part of the peptide (in red)
PTM programme affinity purification

6. ELISA testing of the purified pAb against the non-PTM and PTM peptides
7. Shipping

PTM programme overview



  Speedy Anti-PTM* Classical Anti-PTM *
  Eurogentec designs and synthesises two peptides: one modified with the PTM and one unmodified

Speedy Anti-PTM
Click to enlarge
Eurogentec designs and synthesises two peptides: one modified with the PTM and one unmodified

Classical Anti-PTM
Click to enlarge
Peptide synthesis 1 unmodified peptide
>70% purity
10-13 aa
10-20 mg
1 modified peptide (PTM)
>70% purity
10-13 aa
15-20 mg
1 unmodified peptide
>70% purity
10-13 aa
10-20 mg
1 modified peptide (PTM)
>70% purity
10-13 aa
15-20 mg
Coupling to carrier 5 mg of modified peptide
Coupled to
KLH
BSA
OVA
THY
or MAP carrier
5 mg of modified peptide
Coupled to
KLH
BSA
OVA
THY
or MAP carrier
Available hostb (Number of host immunised) Rabbit (2)
Rat (6)
Guinea-pig (6)
Rabbit (2)
Rat (6)
Guinea-pig (6)
ELISA Guarantee (O) (O)
Affinity purification Double purification of 50 mL serum Double purification of 50 mL serum
QC validation ELISA and SDS-PAGE ELISA and SDS-PAGE
Delivery Purified antibody
Remaining serum
Remaining peptides
Purified antibody
Remaining serum
Remaining peptides
 
Request a quote
Request a quote
 
Order form
Order form
a. See the section "How to provide your antigen"
b. The guarantee only applies for peptides when approved by Eurogentec
*Post Translational Modification
(O) optional

How to start

1. Fill in the request form
2. Receive a quotation (and recommendations for the peptide sequence in case of anti-peptide production)
3. Complete and send the order form (you will receive the link by email with your quotation)
4. Receive a confirmation email (at this time, your immunisation will start)

For a general discussion about your project or a tailor-made quotation, please contact your sales representative or send your request to proteomics.services@eurogentec.com.

We know that confidentiality is significant for you and we handle your data and project with utmost care and respect.


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