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Speedy immunisation programmes

Speedy immunisation programmes

Speedy 28-Day is the reference programme for the production of custom polyclonal antibodies at Eurogentec.

This proprietary programme uses a non-Freund adjuvant that stimulates the immune response and gives mostly IgG’s, despite the short immunisation time. Speedy 28-Day is recommended for:

  • A strong and quick response
  • An anti-PTM programme
  • A quick access to purified and ready to use pAb


Speedy immunisation programme


Antibodies from Speedy 28-Day programme can be generated from peptides designed and synthetised by Eurogentec or from antigens provided by the customers. Speedy 28-Day can be performed in rabbits, rats, Guinea-pigs or goat.

Speedy 28-Day Programmes overview



  Anti-antigen Speedy Mini Anti-peptide Double X Anti-PTM*
  You provide the antigena (peptide, protein, complex sample) Eurogentec designse and synthesises one peptide Eurogentec designse and synthesises one peptide Eurogentec designs and synthesises two peptides to increase the chances of success Eurogentec designs and synthesises two peptides: one modified with the PTM and one unmodified
Peptide synthesise X 1 peptide
>70% purity
10-16 aa
8-10 mg
1 peptide
>70% purity
10-16 aa
15-25 mg
2 peptides
>70% purity
10-16 aa
15-25 mg
1 unmodified peptide
>70% purity
10-13 aa
10-20 mg
1 modified peptide (PTM)
>70% purity
10-13 aa
15-20 mg
Coupling to carrier (O)
KLH
BSA
OVA
THY
or MAP carrier
2-3 mg
coupled to KLH
5 mg
Coupled to
KLH
BSA
OVA
THY
or MAP carrier
5 mg of each peptide
Coupled to
KLH
BSA
OVA
THY
or MAP carrier
5 mg of modified peptide
Coupled to
KLH
BSA
OVA
THY
or MAP carrier
Available hostb (Number of host immunised) Rabbit (2)
Rat (2)
Guinea-pig (2)
Goat (1)
Rabbit (1) Rabbit (2)
Rat (2)
Guinea-pig (2)
Goat (1)
Rabbit (2)
Rat (2)
Guinea-pig (2)
Goat (1)
Rabbit (2)
Rat (6)
Guinea-pig (6)
ELISA Guaranteec X Specific response at 1/20000 No Result, No Serum, No Invoicedd Specific response at 1/20000 No Result, No Serum, No Invoicedd Specific response at 1/20000 (at least for one of the 2 peptides)d (O)
Affinity purification (O) Purification of 10 mL Serum (O) (O) Double purification of 50 mL serum
QC validation (O) ELISA ELISA ELISA ELISA and SDS-PAGE
Delivery Serum
Purified antibody
(O)
Serum
Purified antibody
Serum
Purified antibody
(O)
Remaining peptide
Serum
Purified antibody
(O)
Remaining peptides
Purified antibody Remaining serum Remaining peptides
 
Request a quote
Request a quote
Request a quote
Request a quote
Request a quote
 
Order form
Order form
Order form
Order form
Order form
a. See the section "How to provide your antigen"
b. Other hosts are available on request
c. The guarantee only applies for peptides when approved by Eurogentec
d. The guarantee does not apply for Goat immunisation
e. Please note that peptide design aims at conferring high antigenicity to the peptides, but doesn’t guaranty that they will lead to antibodies functional in any application. To guarantee an optimal functionality of the generated antibodies in your application, we suggest our DoubleX strategy which involves the design and co-injection of two peptides from the same antigen. This co-immunization more closely mimics the protein than a single peptide strategy and has success rates in the 90 % in Western Blots using this technique.
*Post Translational Modification
X not applicable
(O) optional

How to provide your antigen

Antigen (peptide, protein, complex sample) can be provided in solution or lyophilised. Gel slice format is not compatible with the Speedy 28-Day. In that case we recommend our Classical programmes.

The minimal amount of antigen needed depends on the antigen molecular weight and the host as listed in the table below.

Host Antigen amount
Antigen MW<18kDa (µg) Antigen MW>18kDa (µg)
Per animal and per injection Total amount to provide for a Speedy 28-Day Per animal and per injection Total amount to provide for a Speedy 28-Day
Rabbit 200 1600 100 800
Rat 50 400 30 240
Guinea Pig 50 400 30 240
Goat 400 1600 200 800

Speedy More than rapidity

  • Speedy Mini
    The Speedy Mini is designed to give you a quick access to a small quantity of specific custom antibody, purified and hence ready-to-use.

  • Speedy Dx
    To increase the chances of success the Speedy-DX programme uses two peptides, corresponding to spatially distinct regions of the target protein surface. This increases the opportunity of the resultant polyclonals to recognise the target protein in its natural environment. Each host will receive a combination of the two peptides.

  • Speedy Post Translational Modification (PTM)
    Speedy 28-Day includes a very efficient immunisation protocol with frequent boosts. It is therefore well adapted to generate Abs, specifically targeting PTMs, as it can minimise instability of the modification(s) harbored on the peptide used as antigen. By far the most popular requested PTMs specific Abs are directed to phosphorylated residues but we can also do Anti-Methylation, Anti- Acetylation and many more PTM Abs.

How to start

1. Fill in the request form
2. Receive a quotation (and recommendations for the peptide sequence in case of anti-peptide production)
3. Complete and send the order form (you will receive the link by email with your quotation)
4. Receive a confirmation email (at this time, your immunisation will start)

For a general discussion about your project or a tailor-made quotation, please contact your sales representative or send your request to proteomics.services@eurogentec.com.

We know that confidentiality is significant for you and we handle your data and project with utmost care and respect.


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