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TR-FRET

TR-FRET Technology

TR-FRET Custom labeling services and catalog reagents

TR-FRET product

TR-FRET is a combination of Time Resolved Fluorescence (TRF) and Forster's Resonance Energy Transfer (FRET) technologies. TR-FRET like FRET involves an energy transfer between two fluorophores in close proximity but offers the advantages of reducing background noise and providing a higher sensitivity and reliability than the FRET method.

In TR-FRET instead of a fluorescent dye, the donor molecule is a Lanthanide metal.

Lanthanide metals, such as Terbium (Tb) and Europium (Eu), serve as energy donors. Their fluorescence is long-lived and is characterized by a large Stokes shift providing a high signal-to noise ratio due to minimal crosstalk between excitation and emission wavelengths. The delay between excitation and emission detection minimizes prompt fluorescence interference (coming from biological samples).

Applications

  • FRET/TR-FRET substrates for protease activity detection
  • Protease based drug target screening and discovery
  • Protein-peptide interaction
  • Protein-protein interaction

Benefits



TR-FRET Benefits

Our Offer

Custom Services

Our Europium chelate/fluorophore pairs are designed to achieve overlap of the donor emission and the acceptor absorbance spectra. Eu is typically paired with redshifted dyes (allophycocyanine, Cy®5/CyLyte Fluor 5, or HiLyte™Fluor 647/ Alexa Fluor® 647).

  • Protein & peptide – Substrate labeling

    Features:
    • Specific Degree of substitution (DOS)
    • Custom Formulation
    • Custom QC Available ( SDS-PAGE; Mass-spectrometry; Protein Activity)

    Labels:
    • Donor: Europium chelate (Ex 325 nm)
    • Acceptor: HiLyte™ Fluor 647 or CyLyte Fluor 5 (Em 620 nm)

  • Assay Design & development
    • Preparation of substrates labeled with Europium and/or HiLyte™ Fluor 647
    • Validation of assay with inhibitor(s) or biological compounds
    • Detailed step by step assay protocol.


Kits and reagents

  • AnaTag™ labeling kits for TR-FRET

    Conveniently conjugate your own TR-FRET Protein reagents with our amine reactive Europium chelate and HiLyte™ Fluor 647 labeling kits.
AnaTag™ labeling kits for TR-FRET


  • Streptavidin for TR-FRET

    Streptavidin lacks any carbohydrate modification and has a near-neutral pI. Accordingly it has the advantage of much lower nonspecific binding than avidin and is broadly used in various applications . Streptavidin is a tetrameric protein whith each subunit binding a single molecule of biotin. Streptavidin-biotin bond is the strongest known non-covalent interaction with Kd ~10-15 M.

    Detection Reagent Ex/Em (nm) Cat #  
    Streptavidin, Europium labeled 325/620 AS-72252
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    Streptavidin, HiLyte™ Fluor 647 conjugated 649/674 AS-60667
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  • Antibodies for TR-FRET

    HiLyte™ Fluor 647 labeled Antibody & Protein A  Ex/Em (nm) Cat #  
    Mouse Monoclonal anti-His Tag IgG, HiLyte™ Fluor 647 labeled 649/674 AS-61250-H647
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    Mouse Monoclonal anti-GST Tag IgG, HiLyte™ Fluor 647 labeled   649/674 AS-29531-H647
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    Rabbit anti-His Tag IgG, HiLyte™ Fluor 647 labeled   649/674 AS-29673-H647
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    Rabbit anti-Actin (alpha Smooth muscle specific), HiLyte™ Fluor647 labeled 649/674 AS-29553-H647
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    Rabbit anti-Mouse IgG (H+L), HiLyte™ Fluor 647 labeled 649/674 AS-28164-05-H647
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    Rabbit anti-Goat IgG (H+L), HiLyte™ Fluor 647 labeled 649/674 AS-28168-05-H647
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    Goat anti-Rabbit IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor647 labeled 649/674 AS-61056-05-H647
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    Goat anti-Mouse IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor647 labeled 649/674 AS-61057-05-H647
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    Protein A - HiLyte™ Fluor 647 Conjugate 649/674 AS-72237
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    Europium labeled Antibody Ex/Em (nm) Cat #  
    Mouse Monoclonal anti-His tag IgG, Eu labeled 325/620 AS-72251
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    Mouse Monoclonal anti-GST tag IgG, Eu labeled   325/620 AS-72250
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    Goat anti-Rabbit IgG (H+L), highly cross-adsorbed, Eu labeled 325/620 AS-72249
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    Goat anti-Mouse IgG (H+L), highly cross-adsorbed, Eu labeled   325/620  AS-72248
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