PUREfrex™ is the purest cell-free protein synthesis kit based on the PURE (Protein synthesis Using Recombinant Elements) system developed by Professor Takuya Ueda (University of Tokyo).
The PURE system successfully reconstituted the E.coli transcription/translation process in the test tube.
- PURE: Reducted level of contamination
PUREfrex™ is advanced in the purity by improving the preparation method of ribosomes, tRNAs and all protein constituents compared to the original PURE system. As a result, the contaminating lipopolysaccharide [LPS] from E.coli is reduced below 0.1EU per µL of reaction [Fig.1], and other contaminants like RNase and ß-galactosidase are also reduced. Therefore, PUREfrex™ is perfectly adapted to any downstream application.
Fig.1 Lipopolysaccharides [LPS] activity detected in reaction samples using the PUREfrex™ kit vs competitor kits. The contaminating LPS activity detected in the PUREfrex™ sample is below 0.1 EU/µL.
- EASY: Any tag for your protein purification
Since PUREfrex™ protein components are free of tag, you can fuse your protein of interest to any tag to ease purification step [Fig.2].
Fig.2: His-tagged GFP production using PUREfrex™.
1: Synthesis reaction sample
2: Flowthrough sample
3: Washing step sample
4: Purified His-tagged GFP sample
- FAST: Quick protein production and purification
With PUREfrex™, obtain the expression product of a genetic sequence only takes 2-4 hours.
- FLEXIBLE: Synthesis of various proteins
Because PUREfrex™ only contains fully characterized components, it allows an easy adjustment of the assay conditions.
With PUREfrex™, Eukaryotic and Prokaryotic proteins can be easily produced [Fig.3] from plasmid or PCR templates.
Fig.3 SDS-PAGE of Eukaryotic* and Prokaryotic** protein samples produced using PUREfrex™ : -DNA= negative control, GFP*= Green fluorescent protein, DHFR** = Dihydrofolate reductase, GST* = Glutathione S-transferase, ß-Gal** = ß-Galactosidase, bR** = Bacteriorhodopsin, Luc* = Luciferase, CS* = Citrate synthase, MDH* = Malate dehydrogenase, Her2d1* = Domain1 of Her2
PUREfrex™ is developed for in vitro research use only. PUREfrex™ should not be used for the therapy, diagnostic or administration to animals including human and should not be used as food or cosmetics etc.
For information concerning commercial use of PUREfrex™, please contact GeneFrontier: firstname.lastname@example.org.
PUREfrex™ is a trademark of GeneFrontier Corp.